In this free webinar, learn how to prevent peptide feed impurities from fouling reversed phase chromatography (RPC) columns. Attendees will learn how introducing a cation exchange chromatography (CIEX) step upstream of high-performance silica-based RPC reduces the burden from impurities and enhances target peptide purity. Attendees will also learn how a variety of peptide modifications that cannot be removed by RPC can be separated by ion exchange chromatography. The featured speaker will discuss examples of two solid-phase synthesized peptides and one peptide expressed in Escherichia coli, each purified with an upstream CIEX step before one or two RPC steps.
(PRWeb April 28, 2022)
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